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m13 mp18 ss  (TaKaRa)


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    TaKaRa m13 mp18 ss
    M13 Mp18 Ss, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 81 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m13 mp18 ss/product/TaKaRa
    Average 94 stars, based on 81 article reviews
    m13 mp18 ss - by Bioz Stars, 2026-03
    94/100 stars

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    HPLC analysis of an extract from an 11.5-day-old C57BL/6 mouse embryo using the dNTP confirmation assay. ( A ) The two chromatograms show a mock-treated sample and a sample treated with <t>DNA</t> polymerization reagents (DNApol). <t>M13</t> DNA, primers and DNA polymerase were present only in the DNApol sample, but the treatments were otherwise identical. The arrows in the bottom chromatogram show the specific removal of dNTPs. Peaks c and g are unknown components in the cellular extracts. ( B ) The same chromatograms as in A but shown at a lower magnification to visualize the rNTPs used as internal loading controls. The Fast Protocol was used for the HPLC analysis.
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    m13 mp18 ss  (TaKaRa)
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    HPLC analysis of an extract from an 11.5-day-old C57BL/6 mouse embryo using the dNTP confirmation assay. ( A ) The two chromatograms show a mock-treated sample and a sample treated with <t>DNA</t> polymerization reagents (DNApol). <t>M13</t> DNA, primers and DNA polymerase were present only in the DNApol sample, but the treatments were otherwise identical. The arrows in the bottom chromatogram show the specific removal of dNTPs. Peaks c and g are unknown components in the cellular extracts. ( B ) The same chromatograms as in A but shown at a lower magnification to visualize the rNTPs used as internal loading controls. The Fast Protocol was used for the HPLC analysis.
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    HPLC analysis of an extract from an 11.5-day-old C57BL/6 mouse embryo using the dNTP confirmation assay. ( A ) The two chromatograms show a mock-treated sample and a sample treated with <t>DNA</t> polymerization reagents (DNApol). <t>M13</t> DNA, primers and DNA polymerase were present only in the DNApol sample, but the treatments were otherwise identical. The arrows in the bottom chromatogram show the specific removal of dNTPs. Peaks c and g are unknown components in the cellular extracts. ( B ) The same chromatograms as in A but shown at a lower magnification to visualize the rNTPs used as internal loading controls. The Fast Protocol was used for the HPLC analysis.
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    HPLC analysis of an extract from an 11.5-day-old C57BL/6 mouse embryo using the dNTP confirmation assay. ( A ) The two chromatograms show a mock-treated sample and a sample treated with DNA polymerization reagents (DNApol). M13 DNA, primers and DNA polymerase were present only in the DNApol sample, but the treatments were otherwise identical. The arrows in the bottom chromatogram show the specific removal of dNTPs. Peaks c and g are unknown components in the cellular extracts. ( B ) The same chromatograms as in A but shown at a lower magnification to visualize the rNTPs used as internal loading controls. The Fast Protocol was used for the HPLC analysis.

    Journal: Nucleic Acids Research

    Article Title: Isocratic HPLC analysis for the simultaneous determination of dNTPs, rNTPs and ADP in biological samples

    doi: 10.1093/nar/gkab1117

    Figure Lengend Snippet: HPLC analysis of an extract from an 11.5-day-old C57BL/6 mouse embryo using the dNTP confirmation assay. ( A ) The two chromatograms show a mock-treated sample and a sample treated with DNA polymerization reagents (DNApol). M13 DNA, primers and DNA polymerase were present only in the DNApol sample, but the treatments were otherwise identical. The arrows in the bottom chromatogram show the specific removal of dNTPs. Peaks c and g are unknown components in the cellular extracts. ( B ) The same chromatograms as in A but shown at a lower magnification to visualize the rNTPs used as internal loading controls. The Fast Protocol was used for the HPLC analysis.

    Article Snippet: For the DNA polymerase assay, we used M13 mp18 ss-DNA (250 μg/ml) from New England Biolabs, Ipswich, MA, USA (#N4040S), random decamers from Thermo Fischer Scientific (#AM5722G), and Klenow Fragment Exo – (5 U/μl) from Thermo Fischer Scientific (#EP0421).

    Techniques: